Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease
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چکیده
منابع مشابه
Handicap-Recover Evolution Leads to a Chemically Versatile, Nucleophile-Permissive Protease
Mutation of the tobacco etch virus (TEV) protease nucleophile from cysteine to serine causes an approximately ∼104 -fold loss in activity. Ten rounds of directed evolution of the mutant, TEVSer , overcame the detrimental effects of nucleophile exchange to recover near-wild-type activity in the mutant TEVSer X. Rather than respecialising TEV to the new nucleophile, all the enzymes along the evol...
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ژورنال
عنوان ژورنال: ChemBioChem
سال: 2015
ISSN: 1439-4227
DOI: 10.1002/cbic.201500295